Fig. 5
TRIM27 promotes growth and tumorigenicity of tumor cells. a Effects of TRIM27 on cell anchorage-independent growth. The control and TRIM27-overexpressing RKO cells (2 × 103) were seeded in soft agar in 6-well plates. After 3 weeks, the colonies were photographed (left panel), and colony numbers were counted in each well (right panel). Results are represented as mean ± SD, n = 3. Scale bars, 3 mm. b TRIM27 promotes tumor growth in nude mice. The control and TRIM27-overexpressing RKO cells (1 × 107) were injected into the flanks of nude mice. Mice were killed and photographed at 16 days after injection and tumor weights (upper panel) were measured. The tumor sizes (lower panel) were measured at an interval of 4 days after injection and calculated as =π/6 × L × W × H, where L, W, and H represent the length, width, and height of the tumor, respectively. Results are represented as mean ± SD, n = 7. Scale bars, 1 cm. c Effects of TRIM27 knockdown on cell anchorage-independent growth. The control and TRIM27-RNAi RKO cells (2 × 103) were seeded in soft agar in 6-well plates. After 3 weeks, the colonies were photographed (left panel), and colony numbers were counted in each well (right panel). Results are represented as mean ± SD, n = 3. Scale bars, 3 mm. d Knockdown of TRIM27 inhibits tumor growth in nude mice. The control and TRIM27-RNAi RKO cells (1 × 107) were injected into the flanks of nude mice. Mice were killed and photographed at 16 days after injection and the tumor weights (left panel) were measured. The tumor sizes (right panel) were measured at an interval of 4 days after injection and calculated as V = π/6 × L × W × H, where L, W, and H represent the length, width, and height of the tumor, respectively. Results are represented as mean ± SD, n = 5. Scale bars, 1 cm. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t test. Data shown are from a representative experiment repeated for at least twice.
