Fig. 7
Deficiency of TRIM27 in hematopoietic cells is responsible for attenuation of DSS-induced colitis. a Four groups of mice (WT > WT, WT > KO, KO > WT, and KO > KO) generated by bone marrow transplantations were treated with 3% DSS over a 9-day period, and their body weights were daily monitored. Results are represented as mean ± SD, n = 10–12. b Survival of mice described in a was monitored until the 13th day. Results are represented as mean ± SD, n = 10–12. c Colon lengths of mice described in a were measured 9 days after 3% DSS treatment. Results are represented as mean ± SD, n = 7–12. d Representative images of haematoxylin and eosin staining of colon tissues of mice described in c. Scale bars, 100 μm. e Histological analysis of colon tissues described in d. The histological scores were determined in a double-blind manner. Results are represented as mean ± SD, n = 5. f Immunoblotting analysis of pY705-STAT3 and p-P65 levels in colon tissues of mice described in c, n = 3. g qPCR analysis of colon tissues of mice described in c. Results are represented as mean ± SD, n = 3. h ELISA measurement of cytokine levels in sera and colon tissues of mice described in a. Results are represented as mean ± SD, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t test (a, c, e, g, h) or log-rank test (b). Data are representative of two experiments with similar results
