Fig. 3

Re-directed differentiation of MHC-II selected thymocyte by loss of the E4m enhancer. a, b Dot plots in a showing CD4 and CD8 expression in indicated cell subsets from B2m^−/−: Cd4^+/+ and B2m^−/−: Cd4^ΔE4m/ΔE4m mice. Graphs in b showing summary of percentage of CD4⁻CD8⁺ cells in mature (CD24^loTCRβ^hi) thymocytes and the lymph node (LN) TCRβ⁺ population. Means ± SD. **p < 0.01, ***p < 0.001 (unpaired student t test, two-sided). c Histogram at left showing Thpok-gfp expression in freshly-selected (CD24^hiTCRβ^hi) thymocytes of B2m^−/−: Cd4^+/+ and B2m^−/−: Cd4^ΔE4m/ΔE4m mice. Histogram at right showing CD8 expression in Thpok-gfp^lo and Thpok-gfp^hi CD24^hiTCRβ^hi thymocytes of B2m^−/−: Cd4^ΔE4m/ΔE4m mice. One representative result of two mice. d Dot plots showing CD4 and CD8 expression three and five days after TCR stimulation of sorted CD4⁻CD8⁺ mature thymocytes from B2m^+/−: Cd4^+/ΔE4m and B2m^−/−: Cd4^ΔE4m/ΔE4m mice. Numbers in quadrants indicate respective cell percentages. e IL7R expression in CD4⁻CD8⁺ thymocytes (left) and phosphorylated Stat5 levels in CD4⁻CD8⁺ thymocytes (right) at 5 and 15 min following IL7 stimulation at indicated concentrations from B2m^+/−: Cd4^+/ΔE4m and B2m^−/−: Cd4^ΔE4m/ΔE4m mice are shown. Numbers in left histograms indicate mean fluorescence intensity (MFI)