Fig. 4
From: Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene
Requirement for Bcl11b in activating Cd4 at two stages. a ChIP-Seq tracks at the Cd4 gene showing binding of Bcl11b in total thymocytes. b ChIP-qPCR analyses for Runx (left) and Bcl11b (right) bindings to the Cd4 proximal enhancer (E4p), Cd4 silencer (S4), and Cd4 maturation enhancer (E4m) in DP thymocytes, peripheral CD4+,and CD8+ T cells. N.C.: negative control region. Summary of three independent ChIP experiments. Means ± SD. *p < 0.05 (One-way ANOVA and Tukey’s multiple comparison test). c Contour plots showing CD4 and CD8 expression in indicated thymocyte subsets of Bcl11b+/+ and Bcl11bm/m newborn mice. Numbers in quadrants indicate respective cell percentages. Representative results of at least three independent analyses. d Summary of percentage of indicated cell types in indicated cell populations of Bcl11b+/+ and Bcl11bm/m mice. Means ± SD. ***p < 0.001 (unpaired student t test, two-sided). e Contour plots showing CD4 and CD8 expression in indicated thymocyte subsets and lymph node (LN) T cells of mice with indicated genotypes. Numbers in quadrants indicate respective cell percentages. Representative results of at least three independent analyses. f Statistical summary of percentage of CD4+ cells in CD8− mature thymocytes and CD8− lymph node (LN) T cells of Cd4ΔE4p/ΔE4p mice and Cd4ΔE4p/ΔE4p: Bcl11bF/F: Cd4-Cre mice mice. Means ± SD. ***p < 0.001 (unpaired student t test, two-sided). g Dot plots showing CD4 expression and cell divisions five days after in vitro stimulation of sorted CD4+ T cells from mice with indicated genotypes. One representative of three experiments
