Fig. 7

ThPOK is necessary for Runx-mediated repression of E4m activity. a Flow cytometry analyses for CD4, CD8 and Thpok-gfp expression during T cell development in Cd4^{ΔE4p:S4M/ΔE4p:S4M} mice in the presence or absence of ThPOK. Numbers in quadrants, indicated gate, and the region in histograms indicate respective cell percentages. b Summary of percentage of CD4⁺ cells in CD8⁺ mature thymocytes and CD8⁺ splenic T cells of Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Thpok^+/gfp and Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Thpok^gfp/gfp mice. Means ± SD. *** p < 0.001 (unpaired student t test, two-sided). c Histogram showing CD4 expression in freshly selected (CD24^hiTCRβ^hi) thymocytes of Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Runx1/Runx3^+/+ and Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Runx1/Runx3^ΔV/ΔV mice. One representative of two independent analyses. d Graph showing summary of three independent ChIP-qPCR experiments for Runx bindings to E4m in pre-selection DP thymocytes from Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Thpok^+/gfp and Cd4^{ΔE4p:S4M/ΔE4p:S4M}: Thpok^gfp/gfp mice. Means ± SD