Fig. 8
From: Apolipoprotein A-IV binds αIIbβ3 integrin and inhibits thrombosis
Intravenous infusion of recombinant apoA-IV inhibits thrombus growth and vessel occlusion in vivo. a Thrombus growth (white) in wild-type mice mesenteric arterioles was inhibited by infusion of recombinant mouse apoA-IV (rM-apoA-IV; n = 13) but not double D mutant apoA-IV (rM-DM; n = 11). Representative images of thrombus growth in all mice tested is shown. b ApoA-IV prevented stable occlusion in a mouse FeCl3 injury carotid artery thrombosis model. The time to first (left) and stable (middle) occlusion was significantly prolonged and the flow index (right) increased (indicating less decrease in blood flow) by infusion of rM-apoA-IV as compared to vehicle (control) or rM-DM (n = 10). Individual experimental points are shown along with median and interquartile range. In the control group, a circle and diamond with thick blue borders identify two cases that gave a value outside of the 90th percentile (red filling) for at least one of the parameters measured; note that abnormalities in the measured parameters are not concordant. In the apoA-IV WT treatment (i.e., rM-apoA-IV) group, a blue and a yellow square identify the two cases in which treatment caused the least change in the blood flow index, highlighting that one of them failed to achieve stable occlusion; thus, delayed stable occlusion is the most sensitive parameter to discriminate the antithrombotic effect of rM-apoA-IV infusion. All significantly different comparisons are shown. apoA-IV WT recombinant mouse apoA-IV, apoA-IV mutant double D mutant apoA-IV. Non-parametric Kruskal–Wallis one-way analysis of variance for multiple paired comparisons. Mean ± SEM. NS not significant, *P < 0.05, **P < 0.01 and ***P < 0.001. Scale bars: 10 μm (a)
