Fig. 2
HSPC-pDCs elicit a precursor-pDCs phenotype. a Phenotypic analysis of pDC-associated markers on HSPC-pDCs at day 21 using Flow Cytometry. For gating strategy, see Supplementary Fig. 2a, b. b Surface expression levels of pDC-related markers on enriched HSPC-pDCs at day 21 in comparison to blood pDCs. c Graphical illustration of the experimental setup of removal of growth factors for 24 h after the 21-day differentiation. After enrichment of HSPC-pDCs at day 21, cells were depleted of growth factors (except IL-3). After 24 h (day 21 + 1) HSPC-pDCs were harvested for phenotypic or functional analyses. d Phenotypic comparison of HSPC-pDCs at day 21 or day 21 + 1. e, f Levels of type I IFN (e) or IL-6 (f) after stimulation with TLR7 or TLR9 agonists of enriched HSPC-pDCs cells at day 21, HSPC-pDCs Day 21 + 1IL-3, or blood pDCs. g Levels of type I IFN after stimulation with agonists directed at cytosolic DNA and RNA sensors of enriched HSPC-pDCs cells at day 21, HSPC-pDCs Day 21 + 1IL-3, or blood pDCs. Data shown are ± SEM of three donors. Statistical analysis was performed using regular two-way ANOVA (b, d–g) followed by Bonferroni post hoc test
