Fig. 2
Menin KO CD8 T cells rapidly proliferate and acquire effector functions. a The results of the immunoblot analysis of the phospho-Akt (Ser473 or Thr308) and total Akt protein in the WT or menin KO activated CD8 T cells. WT or menin KO naive CD8 T cells were stimulated with anti-TCR-β mAb plus anti-CD28 mAb for 36 h and subjected to immunoblotting. b The results of the immunoblot analysis of the phospho-mTOR (Ser2448/2481), total mTOR protein and α-tubulin (control) of the cells in a. c The result of the flow cytometry analysis of phospho-ribosomal S6 (Ser235/236 and Ser240/244) and total ribosomal S6 protein in the cells in a. d The cell division between eFluor670-labeled WT or menin KO naive CD8 T cell was compared upon the indicated concentration of anti-TCR-β mAb plus anti-CD28 mAb in the presence of IL-2 for 48 h. e Naive CD8 T cells from the spleen of the WT and menin KO mice were stimulated with anti-TCR-β mAb plus anti-CD28 mAb in the presence of IL-2 for 2 days. The cells were then further expanded with IL-2 for the indicated days. Representative results of the intracellular FACS analysis of IL-2/IFN-γ in the WT and menin KO CD8 T cells on the indicated days. The percentages of cells are indicated in each quadrant. f The results of the intracellular FACS analysis of GzmB in the WT and menin KO CD8 T cells on day 3
