Fig. 4
Metabolic profiling of menin KO activated CD8 T cells. a WT or menin KO naive CD8 T cells were stimulated with anti-TCR-β and anti-CD28 mAbs in the presence of IL-2 for 24 h. 2-NBDG was then added to the cultures for 30 min, and its incorporation was determined by FACS. A representative FACS profile is shown. b The intracellular level of glutamine in naive and activated CD8 T cells with WT or menin KO background are indicated (n = 3: biological replicates). The results are presented with the standard deviation. c The intracellular amount of glucose 6-phosphate (G6P), fructose 1,6-diphosphate (F1, 6P), pyruvate, and lactate in the WT and menin KO activated CD8 T cells are presented with the standard deviation (n = 3: biological replicates). Naive CD8 T cells were stimulated with anti-TCR-β and anti-CD28 mAbs in the presence of IL-2 for 36 h. d The intracellular amount of glutamine and glutamate of the cells in c. The results are presented with the standard deviation (n = 3: biological replicates). e The intracellular amounts of TCA cycle intermediates of the cells in c. The results are presented with the standard deviation (n = 3: biological replicates). f, g Naive CD8 T cells were stimulated with anti-TCR-β and anti-CD28 mAbs in the presence of IL-2 for 36 h, and then glycolysis (f) and the OCR (g) were determined before or 20 min after glucose (10 mM) injection. b, f, g **p < 0.01 (Student’s t-test), c, d, e *p < 0.05, **p < 0.01, ***p < 0.001 (Welch’s t-test)
