Fig. 6
Demethylation of histone H3K27 is involved in CD8 T-cell dysfunction. a The result of the immunoblot analysis of the di- or tri-methylated histone H3K27, tri-methylated histone H3K4 and total histone H3 in the WT CD8 T cells cultured under the indicated conditions for 48 h. The protein amount of histone H3 was used as a loading control. b The results of the immunoblot analysis of the di- or tri-methylated histone H3K27, tri-methylated histone H3K4 and total histone H3 in the WT or menin KO CD8 T cells cultured under normal conditions for 3 days. c A representative staining profile of CD62L/CD27 on the cell surface of the WT, utx KO menin KO or menin/utx-double KO effector CD8 T cells on day 7. The percentages of cells are indicated in each quadrant. d Representative staining profile of PD-1 on the cell surface of the cells in c. e The ELISA results for IL-6, IL-10, OPN, and IFN-γ in the supernatants of the cells in c restimulated with immobilized anti-TCR-β for 16 h are shown with the standard deviation (n = 3: biological replicates). f The results of the quantitative RT-PCR analysis of the pro-inflammatory enzymes of the cells in c. The results are presented relative to the expression of Cd3ε mRNA with the standard deviations (n = 3: technical replicates). g The percentages of SA β-galactosidase (SA β-Gal)-positive cells on day 12 after the initial anti-TCR-β/CD28 stimulation are shown with the standard deviation (n = 3: biological replicates). *p < 0.05, **p < 0.01 (Student’s t-test)
