Fig. 1
Deleting or disrupting HDAC3 ameliorates age-related impairments in hippocampal memory. a OLM procedure. AAV was infused 2 weeks before training. b 18-m.o. HDAC3flox/flox mice showed significantly better memory for OLM compared to wild type (HDAC3+/+) littermates (Two-way ANOVA: Significant Genotype x Session interaction (F(1,29) = 15.96, p < 0.001), Sidak’s post-hoc tests, ***p < 0.001, n = 14(5F), 17(6F)). c Total exploration was similar for both groups at test (t(29) = 1.67, ***p = 0.11). d Disrupting HDAC3 activity in the dorsal hippocampus with AAV-HDAC3(Y298H)-V5 also ameliorated hippocampal memory impairments in 18-m.o. mice (Two-way ANOVA: main effect of session (F(1,16) = 15.96, p < 0.001), Sidak’s post hoc tests, ***p < 0.001, *p < 0.05, n = 9,10; all males). e Total exploration time was similar for both groups at test (t(16) = 0.28, p = 0.78). f ORM experimental procedure, 2 weeks after the completion of OLM. g Both 18-m.o. HDAC3flox/flox mice and HDAC3+/+ littermates showed little preference for the novel object (Two-way ANOVA, no main effects or interaction, n = 14(5 F), 17(6 F)). h Total exploration time was similar for both groups at test (t(29) = 0.59, p = 0.56). i Disrupting HDAC3 activity in the dorsal hippocampus with AAV-HDAC3(Y298H) also had no effect on ORM, with neither group showing preference for the novel object (Two-way ANOVA, no main effects or interaction, n = 9,10; all males). j Groups showed similar total exploration time at test (t(16) = 0.28, p = 0.78). Data are presented as mean ± SEM; black circles, males; gray squares, females
