Fig. 3
CD123+ stem cells exhibit increased translation but no change in cell cycle status. a CD123+ cells(red histogram) vs. CD123- cells(blue histogram) from Lin−/CD34+/CD38− high-risk bone marrow specimens exhibit increased protein synthesis as measured by op-puromycin in 11 independent patient specimens b Ratio of the mean fluorescence of CD123+ vs. CD123- cells shows an average 2-fold increase in CD123+ cells. c Cell cycle analysis of a representative MDS specimen (MDS 4) showing bulk, lin−, lin−/CD34+ and CD123+/CD123− stem cell populations. d Aggregate data comparing cell cycle in eight separate patient specimens shows no significant differences in CD123+ vs. CD123− cells. **p < .05 (two-tail t-test) error bars are S.D
