Fig. 5
CD123 positive cells show differential sensitivity to multiple agents. Viability of primary MDS specimens following overnight culture in the presence of 200 nM omacetaxine, a protein synthesis inhibitor (a), or 200 nM ABT-199, a Bcl2 inhibitor (b). Data gated on CD123- vs. CD123+ primitive populations (Lin−/CD34+/CD38−). c Viability of a typical MDS specimen following overnight culture in varying drug conditions (left panel) in comparison to normal bone marrow CD34+ cells (right panel). ABT-199 (200 nm), OMA (omacetaxine, 200 nM), and Aza (azacitidine, 2.5uM). * indicates p < 0.01; bars represent mean ± S.D. from three replicates. d Six independent patient samples showing toxicity to drugs used in panel c comparing CD123+ vs. CD123- cells. e Protein synthesis levels following 4 h of treatment with omacetaxine,ABT-199, azacitidine, omacetaxine plus ABT-199, or omacetaxine plus azacitidine as indicated by OP-puromycin labeling. *p < .05 (two-tail t-test) error bars are S.D
