Fig. 6

In vivo engraftment of high-risk primary MDS bone marrow specimens. Representative plots from 4 independent high risk MDS patient specimens engrafted in mice showing human CD45 staining (a), CD34/CD38 staining and CD123 staining (b). c Flow cytometry to analyze the immunophenoype of bone marrow specimens pre- and post-engraftment in NSG-S mice. d H&E staining of mouse femur post engraftment shows an abundance of blast-like cells as well as hypolobated megakaryocytes (arrows), indicative of acute disease evolving from antecedent MDS. e Representative plot of OP-puromycin labeling on cells derived from the marrow of xenograft mice. Labeling shown for CD123+ vs. CD123- cells, gated on CD45+/Lin−/CD34+/CD38−. f Aggregate data showing three independent patient xenografts stained for OP-puromycin as in panel e. ** indicates p < 0.01 (two-tail t-test) error bars are S.D