Fig. 4

Histopathology of muscle tissues from ribitol-treated P448L mice. Seven-week-old P448L mice were given drinking water only, or drinking water supplemented with 5% ribitol for either 3 months (3M) or 6 months (6M). a H&E staining of heart, tibialis anterior (TA), and diaphragm tissues from untreated (untreated 3M and untreated 6M) or ribitol-treated (5% ribitol 3M and 5% ribitol 6M) P448L mice, and C57 mice. Arrow indicates areas of heavy infiltration in the control TA muscle. Scale bar, 50 μm. b Fiber size distribution of TA muscles of either untreated (n = 3) or ribitol-treated (n = 6) P448L mutant mice, and C57 (n = 3) mice. c Percentage of CNF in TA muscles of P448L mice treated with 5% ribitol for 3M (n = 6) and 6M (n = 4), or aged-matched untreated (n = 3) P448L mice and C57 (n = 3) mice. Error bars represent mean ± SEM. Unpaired t test, *p ≤ 0.05