Fig. 2
From: Development of an antibody fragment that stabilizes GPCR/G-protein complexes
Crystal structure of Gi1/scFv16 and characterization of Fab16. a Overall structure of Gi1/scFv16 complex. Cartoon representation with Gαi1 in gold, Gβ in cyan, Gγ in magenta, scFv-heavy chain in light grey and scFv-light chain in light blue. b Superposition of Gi1/scFv16 structure onto Gi1 (PDB: 1GP2) based on alignment of Gβγ subunits. Gαi1 (1GP2) in grey and Gi1/scFv16 in the same colour code as in a. For clarity, Gβγ subunits and scFv16 is shown as transparent cartoon. Arrows show a slight rotational displacement of Gαi1 towards Gβ1 compared to Gi1 alone. Additional interactions are formed between switch I and switch II of Gαi1 and Gβ1. c Interaction between Gi1 and scFv16. The residues participating in the interactions are depicted with stick models in the expanded panels. Residue numbers are shown with Common Gα Numbering (CGN) code for Gαi142
