Table 2 Effect of Fab16 or Nb35 on the nucleotide-binding/releasing rate

From: Development of an antibody fragment that stabilizes GPCR/G-protein complexes

 

M2R/GoA + GTPγS

M2R/GoA + Fab16 + GTPγS

M2R/GoA + GDP

M2R/GoA + Fab16 + GDP

k (1/s)

0.061 ± 0.002 (fast)a

0.00092 ± 0.00004

0.00244 ± 0.00003

0.00042 ± 0.00009

k (1/s)

0.0032 ± 0.0002 (slow)a

   
 

Gi1 + GTPγS

Gi1 + Fab16 + GTPγS

Gi1 + GDP

Gi1 + Gab16 + GDP

k (1/s)

0.00206 ± 0.00003

0.00116 ± 0.00003

0.0115 ± 0.0002

0.00572 ± 0.00007

 

β2AR/Gs + GTPγS

β2AR/Gs + Nb35 + GTPγS

β2AR/Gs + GDP

β2AR/Gs + Nb35 + GDP

k (1/s)

0.00327 ± 0.00005

ND

ND

ND

  1. All values are expressed as mean ± s.e.m. of triplicate experiments
  2. aThe binding kinetics was best fit and analysed by two phase association. The fraction corresponding to the fast component is 0.526 ± 0.005
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