Fig. 3

Brg1 expression inversely correlates with FBW7 to govern gastric cancer progression. a The scores of FBW7 immunohistochemical (IHC) staining in 400 tumor sections and paired adjacent non-tumor tissues. b The mRNA expressions of FBW7 and Brg1 were examined by real-time PCR. The data were analyzed by Student’s t test. c, d The scores of Brg1 immunohistochemical staining in tumor sections and paired adjacent non-tumor tissues in total (c) or in different TNM (tumor node metastasis) stages (d). The data were analyzed by Student’s t test. e High Brg1 expression positively correlated with lymph node metastasis in gastric cancer. Their correlation was analyzed by Spearman rank correlation test. f The association of Brg1 expression with overall survival was examined by the Kaplan–Meier analysis in gastric cancer patients from the Zhongshan cohort. g Representative images of FBW7, Brg1, and E-cadherin staining were shown in consecutive normal sections and distant metastatic tumor sections. Scale bar, 100 μm. h, i The correlations between Brg1 and FBW7 levels (h), Brg1 and E-cadherin levels (i) in 400 consecutive tumor sections that were semiquantified as high or low and analyzed by Spearman rank correlation test