Fig. 4

Brg1 promotes cell migration in vitro and metastasis in vivo in gastric cancer cells. a IB analysis of WCLs derived from MKN45 cells infected with the indicated lentiviral shRNA constructs. b, c Representative images of migrated MKN45 cells infected with the indicated lentiviral shRNA constructs in a transwell assay (b) and quantification of migrated cells in (c). Data were shown as mean ± SD of three independent experiments. *p < 0.05, Student’s t test. Scale bar, 100 μm. d The migration of MKN45 cells transfected with the indicated lentiviral shRNA plasmids in scratch experiments. Data were shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, Student’s t test. e–g Representative images and statistical analysis of metastasis formed in lungs of each nude mouse (n = 5) at 2 months after tail vein injection of MKN45 cells (3 × 10⁶) with the indicated lentiviral shRNA plasmids. Scale bar, 1 mm. h IB analysis of whole cell lysates derived from AGS cells transfected with the indicated Flag-Brg1 constructs. i, j Representative images of migrated AGS cells transfected with the indicated Flag-Brg1 constructs in a transwell assay and quantification of migrated cells. Data were shown as mean ± SD of three independent experiments. *p < 0.05, Student’s t test. Scale bar, 100 μm. k The migration of AGS cells transfected with the indicated Flag-Brg1 constructs in scratch experiments. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, Student’s t test. l–n Representative images and statistical analysis of metastases that formed in lungs of each nude mouse (n = 5) at 2 months after tail vein injection of AGS cells (2 × 10⁶) transfected with the indicated Flag-Brg1 plasmids. *p < 0.05, **p < 0.01, Student’s t test. Scale bar, 1 mm