Fig. 2
From: Nucleolar fibrillarin is an evolutionarily conserved regulator of bacterial pathogen resistance
FIB-1/fibrillarin and nucleolar size are reduced upon bacterial infection. a, b FIB-1 levels are significantly reduced in wild-type N2 after S. aureus and E. faecalis infection. FIB-1 is modestly reduced in ncl-1 mutants after infection but the levels are still higher relative to wild-type N2 after infection. Error bars represent mean ± s.e.m. of three independent biological replicates. c FIB-1::GFP shows reduced fluorescent signal after infection with S. aureus. d, e Nucleolar size in hypodermal cells of wild-type N2 worms is reduced after 12-h infection with S. aureus and E. faecalis. Nucleolar size in hypodermal cells of ncl-1 mutants after infection remains unaffected. Error bars represent mean ± s.d. f S. aureus infection for 12 h reduces 26S and 18S rRNA levels in wild-type C. elegans. RNA extracted from equal number of uninfected and infected worms was analyzed using a bioanalyzer. Scale bars represent 20 (c) and 5 μm (d). *P < 0.05, **P < 0.01, ns—non-significant, unpaired t-test
