Fig. 5
Recapitulation of mutant SF3B1-associated abnormal splicing in vitro. a The number of mutant SF3B1-associated events that showed a consistently significant difference in CRISPR cell lines with the SF3B1K700E mutation. Bars on the right indicate alternative splicing events that were more frequently found in SF3B1-mutated cell lines. Bars on the left represent those that were more frequently found in the controls. Red bars indicate events with consistent changes with a P value <0.05 in CRISPR cell lines. b, c Scatter plots showing mean PSI values of mutant SF3B1-associated alternative 3′ splice sites (b) and intron retention (c). The left and right panels show mean PSI values in primary bone marrow samples and in CRISPR cell lines, respectively. d A volcano plot comparing mean PSI values of mutant SF3B1-associated intron retentions between CRISPR cell lines with and without the SF3B1K700E mutation (n = 3 each). X-axis indicates fold changes in PSI values on a log2 scale. Y-axis indicates P values on a negative log10 scale
