Fig. 2
From: Linking prostate cancer cell AR heterogeneity to distinct castration and enzalutamide responses
Four primary (1°) CRPC exhibit distinct changes in AR and other molecules. Four xenograft models were serially passaged in castrated male NOD/SCID (for LAPC9 and LAPC4; a or NOD/SCID-γ (NSG; for LNCaP and VCaP; b mice. The CRPC (passages indicated) and their corresponding AD tumors were harvested for WB analysis of indicated “AR and targets” and “CSCs and castration resistance” molecules (for AR, the clone 441 mouse mAb was used; Supplementary Table 1). PC3 cells were used as negative controls for AR. For each sample, whole cell lysate (60 μg) was loaded each lane, and GAPDH served as loading controls (top and bottom lanes). In a, no AR-V7 was detected in either LAPC9 or LAPC4 models. In b, the bands below AR indicated by a bracket are likely AR variants. *a non-specific band
