Fig. 2

Kinase activation profiling reveals IAV-responsive kinases as potential drug targets. a Top kinases predicted to be involved in the differential phosphorylation of proteins at 5 and/or 15 min post IAV-infection are shown in color (light orange: predicted to be activated at 5 min p.i., red: predicted to be activated at 15 min p.i.; dark red: predicted to be activated at both time points) in the context of all cellular kinases (s significantly enriched hit, t trend towards significance, * top predicted kinase for which enrichment calculation is not possible, see Methods and Supplementary Fig. 3). b Over-represented sequences in IAV-responsive peptides are shown and linked with numbers to the kinases identified in (a). c Effect of inhibitors against the identified IAV-responsive kinases on the replication of a Renilla-encoding virus. The efficiency of viral replication was determined by luminescence measurement (RLUs) in live cells every 2 h. The dotted line represents a 10-fold difference in viral replication with regard to DMSO-treated cells. Error bars represent standard deviation from three replicates