Fig. 5
From: Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
Comparison of the antigenicity properties of membrane-bound and soluble Env trimers. a Median IC50 neutralization and EC50 ELISA titers are provided in µg/ml and colored according to the listed scale for the three HIV-1 strains, including JR-FL E168K, BG505 N332, and B41. Neutralization of the full-length Env-pseudotyped viruses (IC50) by bnAbs was determined in TZM-bl neutralization assays, while binding to the corresponding SOSIP trimers was measured by ELISA-binding assays. For JR-FL E168K, neutralization assays were also performed with pseudovirus grown in the presence of kifunensine (+kif), which blocks glycan processing leaving oligomannose (mainly Man9) glycans at all glycosites. b Flow cytometric measurements for binding of fluorescent bnAb to membrane-bound JR-FL ΔCT Env on 293 F cells grown in the presence and absence of kifunensine. Green (pink, brown, orange, black, blue, or red) solid line represents PG9 (PG16, CH03, PGT145, PGDM1400, PGT128, or PGT151) binding to JR-FL ΔCT (-kifunensine), while green (pink, brown, orange, black, blue, or red) dashed line represents PG9 (PG16, CH03, PGT145, PGDM1400, PGT128, or PGT151) binding to JR-FL ΔCT (+kifunensine), respectively
