Fig. 4
From: Focused clamping of a single neuronal SNARE complex by complexin under high mechanical tension
Kinetic analysis of conformational intermediates through hidden Markov modeling. a Extension time traces measured at 1.2 kHz with a single SNARE complex in the absence (upper) or presence (lower) of 5 μM Cpx. The state paths obtained from hidden Markov modeling are overlaid in red (Supplementary Note 6). Vertical and horizontal scale bars represent 5 nm and 50 ms, respectively. b–e Force dependence of zippering (b, d) and unzipping (c, e) rates for C-terminal SNARE motifs (b, c) and linker domains (d, e) in the absence (black) or presence (blue) of 5 μM Cpx. Data are represented as mean ± s.d. of more than three measurements. Dashed lines represent the fits to the Bell equation. A diagram for the corresponding transitions in a SNARE complex is shown in the center
