Fig. 1 | Nature Communications

Fig. 1

From: Identifying weak interdomain interactions that stabilize the supertertiary structure of the N-terminal tandem PDZ domains of PSD-95

Fig. 1

The PDZ1-2 tandem from PSD-95. a PSD-95 contains five protein-interaction domains connected by linkers of varying length. The N-terminal PDZ tandem contains the first two PDZ domains (residues 61-249). b Structural models of the PDZ tandem determined with different experimental methods. PDZ1 (gray) from each model was aligned to highlight conformational differences. PDZ2 is colored according the method used to resolve the structure. Models from the crystal structure (blue and cyan) [PDB ID: 3GSL]. Model from NMR based on residual dipolar coupling, which was kindly provided by M. Zhang (orange). Model from smFRET (white). c Cysteine mutations used for fluorescent labeling in the PDZ tandem of PSD-95 (Residues 60-249). The first residue is in PDZ1 while the second is in PDZ2. Each pair forms a single distance restraint that is measured independently. d Network of FRET restraints in the PDZ Tandem. Mean position of each dye (spheres) at the associated cysteine residue as determined by modeling. PDZ1 sites colored green. PDZ2 sites colored red. Black lines are shown between mean positions of dyes

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