Fig. 1
From: LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L
Deletion of HOIP or HOIL-1 in keratinocytes results in lethal dermatitis. a Representative images of mice of the indicated genotypes (n = 10 mice per genotype) at P4. b Representative images of skin sections stained with H&E, the indicated antibodies or TUNEL from mice of the indicated genotypes (n = 3 mice per genotype). Arrows: pyknotic nuclei, stars: immune cell infiltrates, arrowhead: parakeratosis and black bar: hyperkeratosis. Nuclei were stained with DAPI (blue). White dashed lines indicate boundary of epidermis (above) and dermis (below). Scale bar, 50 µm. c Epidermal thickness quantification at P4. Data are presented as mean values ± s.e.m. (n = 3 per genotype). ***P ≤ 0.001. d Percentages of CD45+ cells in the skin of the indicated genotypes at P4. Data are presented as mean values ± s.e.m. (n < 8 per genotype), *P ≤ 0.05, ***P ≤ 0.001. e Quantification of TUNEL- and CC3-positive cells as represented in b. Data are presented as mean values ± s.e.m. (n = 3 mice per genotype), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Control mice represent a pool of Hoipfl/fl;K14-Cre− and Hoipfl/wt;K14-Cre+ (white circles) or Hoil-1fl/fl;K14-Cre− and Hoil-1fl/wt;K14-Cre+ (grey circles). P: postnatal day
