Fig. 3
From: LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L
Cell death precedes the onset of inflammation in HoipE-KO and Hoil-1E-KO mice. a Representative images of skin sections stained with H&E, the indicated antibodies or TUNEL from mice of the indicated genotypes (n = 3 mice per genotype). Arrows: pyknotic nuclei, stars: immune cell infiltrates. Nuclei were stained with DAPI (blue). White dashed lines indicate boundary of epidermis (above) and dermis (below). Scale bar, 50 µm. b Quantification of TUNEL- and cleaved caspase-3 (CC3)-positive cells in skin sections from mice of the indicated genotypes. Data are presented as mean values ± s.e.m. (n < 4 mice per genotype). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. c Percentages of CD45+ cells in the skin of the indicated genotypes at the indicated stages. Data are presented as mean values ± s.e.m. (n< 3 per genotype). **P ≤ 0.01, ***P ≤ 0.001, NS: not significant. Control mice represent a pool of Hoipfl/fl;K14-Cre− and Hoipfl/wt;K14-Cre+ (white circles) or Hoil-1fl/fl;K14-Cre− and Hoil-1fl/wt;K14-Cre+ (grey circles). P: postnatal day
