Fig. 5
From: LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L
Adult HoipE-KO mice suffer from cell death-induced dermatitis. a Representative images of mice of the indicated genotypes (n = 8 mice per genotype). Animals were treated with vehicle or 4-OHT every other day for a total of four doses. b Quantification of TUNEL-positive cells in skin sections of Hoipfl/flK14CreERtam mice after one, two, three or four treatments with vehicle or 4-OHT. Data are presented as mean values ± s.e.m. (n = 3 per genotype). ***P ≤ 0.001, NS: not significant. c Quantification of CD45 staining in skin sections from Hoipfl/flK14CreERtam mice treated as in b was performed by measuring overall fluorescence intensity using ImageJ. Results are presented as mean values ± s.e.m. (n = 3 mice per genotype). *P ≤ 0.05, NS: not significant. au: arbitrary units. d Representative images of skin sections stained with H&E, the indicated antibodies and TUNEL from mice of the indicated genotypes (n = 3 mice per genotype) after four 4-OHT treatments. Arrows: pyknotic nuclei, stars: immune cell infiltrates, arrowhead: parakeratosis and black bar: hyperkeratosis. Nuclei were stained with DAPI (blue). White dashed lines indicate boundary of epidermis (above) and dermis (below). Scale bars, 50 µm
