Fig. 5 | Nature Communications

Fig. 5

From: Cytoplasmic localization of GRHL3 upon epidermal differentiation triggers cell shape change for epithelial morphogenesis

Fig. 5

Localization of GRHL3, VANGL2, SCRIB, and β-catenin in LM-epidermal cells. a Immunofluorescence for GRHL3 (green), anti–HaloTag-VANGL2 (red), TROMA-1 (magenta), and DAPI (blue) in an LM-epidermal cell. b Intensity of merged confocal images obtained by GRHL3, VANGL2, and DAPI staining is graphically depicted (a white arrow in a). Asterisks indicate co-localization of GRHL3 and VANGL2 in the cytoplasm. c Immunofluorescence for SCRIB (green), TROMA-1 (magenta), and DAPI (nuclei; blue) in an LM-epidermal cell. d Immunofluorescence for non-muscle myosin light chain (pMLC; green), TROMA-1 (magenta), and DAPI (nuclei; blue) in an LM-epidermal cell. e Immunofluorescence for GRHL3 (green), anti–HaloTag–β-catenin (red), TROMA-1 (magenta), and DAPI (blue) in an LM-epidermal cell. f Intensity of GRHL3, β-catenin, and DAPI in merged confocal images is graphically depicted (a white arrow in e). Representative images from three independent experiments. Scale bars represent 50 μm

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