Fig. 1 | Nature Communications

Fig. 1

From: USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy

Fig. 1

USP24 level in M2 macrophages. a Representative images of immunohistochemistry staining of USP24 in lung cancer specimens by using anti-USP24 antibody. Leukocytes were circled with red dashed lines. Scale bar represents 200 μm. b Immunofluorescence staining of USP24, CD68, and DAPI in one human lung cancer specimen. Scale bar represents 50 μm. c Morphological change of M2 macrophages derived from THP-1 monocytes. Scale bar represents 200 μm. d, e USP24 protein (n = 3) (d) and RNA (n = 3) (e) level in THP-1 and M2 macrophages were analyzed by western blotting and RT-PCR. Results were normalized with tubulin or GAPDH level and expressed as fold of control. Data are shown as mean ± SEM, two-tailed unpaired Student’s t-test, *P < 0.05 and **P < 0.01

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