Fig. 2

Validation of TRAP clients based on Gene Ontology enrichment factors. The color coding follows Fig. 1. Protein annotations of signal peptides, membrane location, and N-glycosylation in humans were extracted from UniProtKB, and used to determine the enrichment of Gene Ontology annotations among the secondarily affected proteins. a, b Summaries of two Sec61 (a) and three TRAP depletion experiments (b), performed in triplicate in each case