Table 1 Primary structures of signal peptides of putative TRAP clients

From: Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import

UniProt ID

Gene name

Signal peptide

TM

N-glyc

GP %

Hph

Q9UM22 *

EPDR1

MPGRAPLRTVPGALGAWLLGGLWAWTLCGLCSLGAVG

−

+

29.7

0.727

Q9H6X2 *

ANTXR1

MATAERRALGIGFQWLSLATLVLICAG

+

+

15.6

0.262

Q8IWB1

ITPRIP

MAMGLFRVCLVVVTA

−

+

6.7

1.951

O00622

CYR61

MSSRIARALALVVTLLHLTRLALS

−

−

0

1.017

P54802

NAGLU

MEAVAVAAAVGVLLLAGAGGAAG

−

+

21.7

1.461

Q9GZX9

TWSG1

MKLHYVAVLTLAILMFLTWLPESLS

−

+

4.0

1.269

Q13454

TUSC3 ***

MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGG

+

−

24.4

−0.01

Q9Y3A6 */**

TMED5

MGDKIWLPFPVLLLAALPPVLLPGAAG

+

−

29.6

1.074

Q9H0U3

MAGT1

MAARWRFWCVSVTMVVALLIVCDVPSASA

+

+

3.5

1.227

Q13214

SEMA3B

MGRAGAAAVIPGLALLWAVGLGSA

−

+

25.0

1.106

Q9BRR6 *

ADPGK

MALWRGSAYAGFLALAVGCVFL

−

−

13.6

1.356

P02751

FN1

MLRGPGPGLLLLAVQCLGTAVPSTGA

−

+

25.8

0.161

P45877 */**

PPIC

MGPGPRLLLPLVLCVGLGALVFSSGAEG

−

+

32.1

1.063

Q9UMX5

NENF

MVGPAPRRRLRPLAALALVLALAPGLPTARA

−

−

22.6

0.281

O14773

TPP1

MGLQACLLGLFALILSGKCSY

−

+

15.8

1.526

P15941 *

MUC1

MTPGTQSPFFLLLLLTVLTVVTG

+

+

17.4

1.310

Q15582

TGFBI

MALFVRLLALALALALGPAATLA

−

−

8.7

1.729

O75629

CREG1

MAGLSRGSARALLAALLASTLLALLVSPARG

−

+

12.9

0.880

Q9ULF5 *

SLC39A10

MKVHMHTKFCLICLLTFIFHHCNHC

+

+

0

0.707

Q08380

LGALS3BP

MTPPRLFWVWLLVAGTQG

−

+

22.2

0.523

P08069 *

IGF1R

MKSGSGGGSPTSLWGLLFLSAALSLWPTSG

+

+

26.7

0.404

P08572 *

COL4A2

MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLA

−

+

20.0

−0.04

Q8N2U0 *

TMEM256

MAGPAAAFRRLGALSGAAALGFASYGAHG

+

−

24.1

0.292

Q9UBV2 *

SEL1L

MRVRIGLTLLLCAVLLSLASA

+

+

4.8

1.651

Q969V3

NCLN

MLEEAGEVLENMLKASCLPLGFIVFLPAVLLLVAPPLPAADA

+

+

16.7

1.038

O14672 *

ADAM10

MVLLRVLILLLSWAAGMG

+

+

15.8

1.775

P11117 */**

ACP2

MAGKRSGWSRAALLQLLLGVNLVVMPPTRA

+

+

16.7

0.304

P06756 *

ITGAV

MAFPPRRRLRLGPRGLPLLLSGLLLPLCRA

+

+

29.7

0.163

Q12907

LMAN2

MAAEGWIWRWGWGRRCLGRPGLLGPGPGPTTPLFLLLLLGSVTA

+

+

31.8

0.230

P56937

HSD17B7

MRKVVLITGASSGIGLALCKRL

+

+

19.1

1.733

P39656 */**

DDOST ***

MGYFRCARAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCA

+

−

14.3

0.115

Q8TB61

SLC35B2

MDARWWAVVVLAAFPSLGAG

+

−

23.8

0.627

Q6PIU2

NCEH1

MRSSCVLLTALVALA

−

+

14.3

1.617

Q5JPE7

NOMO2

MLVGQGAGLLGPAVVTAAVVLLLSGVGPAHG

+

+

29.0

1.259

P08236

GUSB

MARGSAVAWAALGPLLWGCALG

−

+

22.7

0.883

P00533 *

EGFR

MRPSGTAGAALLALLAALCPASRA

+

+

16.7

0.634

Q5VW38

GPR107

MAALAPVGSPASRGPRLAAGLRLLPMLGLLQLLAEPGLG

−

+

28.2

0.538

QBN129 *

CNPY4

MGPVRLGILLFLFLAVHEAWA

−

−

14.3

1.312

  1. Amino acid sequences of signal peptides (SP) are shown together with protein accession number, gene name, presence of transmembrane domains (TM) or N-glycosylation sites (N-glyc) in the mature domain, GP content of SP in %, and SP hydrophobicity (Hph). Signal peptides are divided into N-terminal, hydrophobic, and C-terminal domains according to Phobius prediction (www.phobius.sbc.su.se). According to predictions with the TMHMM server 2.0 (www.cbs.dtu.dk/services/TMHMM/), nine of the precursor polypeptides with SP comprise one transmembrane region in their mature domain, seven of which are type I membrane proteins, i.e. expose their mature N-terminus to the ER lumen or extracellular space56; ten of the precursor polypeptides with SP comprise more than one transmembrane region in their mature domain. Thus, a total of nineteen TRAP dependent precursor polypeptides with cleavable SP, or 50%, are membrane proteins. Notably, PPIC was not listed in Supplementary Table 5 because UniProtKB does not name it as a precursor with SP. However, PPIC has SP according to SignalP 4.1 server (www.cbs.dtu.dk/services/SignalP/). *, accession numbers of proteins, which were also negatively affected by Sec61 complex depletion; **, validated proteins; ***, OST subunits. The DDOST gene codes for Ost48
Back to article page