Fig. 2

EVI1 overexpression suppresses erythropoiesis. a Quantification of erythroid (TER119+) cells overexpressing EVI1 (GFP−) in bone marrow of competitively transplanted recipients harvested 2, 6, or 10 after DOX induction. (WT n = 4 mice, EVI1 n = 4 mice at each time point, total n = 24). b–d Numbers of donor-derived reticulocytes (GFP−, Ter119+, CD71+), erythrocytes (GFP−,Ter119+, CD71−), and platelets (GFP−, low SSC/FSC, CD41+) as indicated at 2, 6, and 10 weeks post induction per microliter of peripheral blood of mice competitively transplanted with DOX-induced Evi1^TO, Rosa26^rtTA mice or WT cells. (WT n = 4 mice, EVI1 n = 4 mice at each time point, total n = 24). e Quantification of apoptotic (annexin-v+ 7AAD−) erythroid (TER119+) cells overexpressing EVI1 (GFP−) or WT WBM of competitively transplanted recipients, expressed relative to WT GFP− cells from the control transplant, harvested 10 weeks after DOX induction. Each dot represents an individual recipient mouse. (WT = 8 mice, EVI1 = 8 mice). f, g Quantification of total (f) and BrdU-positive (g) proerythroblasts (TER119+^low cKit+), and erythroblasts (TER119+ CD71+) in WBM of Evi1^TO/TO Rosa26^rtTA/rtTA mice harvested 48 h with or without DOX induction. (WT n = 4, EVI1 n = 4). For all panels, error bars represent standard deviation; p values calculated with Student’s t-test