Fig. 1 | Nature Communications

Fig. 1

From: Ring synthetic chromosome V SCRaMbLE

Fig. 1

Ring_synV SCRaMbLE to generate genomic and phenotypic variation. The ring_synV (orange circle) was divided into 170 segments (green arrows) by loxPsym sites, in which genes were demonstrated in purple and blue arrows. a The PDV biosynthesis pathway was incorporated in YEL063C (CAN1) of ring_synV by homologous recombination to generate the initial PDV producer strain yWJS1. b The SCRaMbLE system of yWJS1 functioned and SCRaMbLEd colonies with diversity of PDV improvement were isolated when exposed to galactose and estradiol. c Genomic SV analysis of SCRaMbLEants revealed chromosome number variation, CNV and novel SV. d Colonies with increased PDV production were selected when transformed novel SVs into the initial strain

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