Fig. 4 | Nature Communications

Fig. 4

From: Ring synthetic chromosome V SCRaMbLE

Fig. 4

Functional novel SVs that may correlate with PDV production improvement. a Schematics showing novel SV that may correlate with PDV production improvement. The left DNA segment (orange arrow) and right DNA segment (blue) are divided by loxPsym sites (green diamond). The DNA sequence was amplified by using primers designed (black arrow) and transformed into yWJS1 and assembled with linearized pRS413 backbone plasmid. We isolated transformants with virtual darker colors by selection from 11 novel SVs than the initial strain. b Fold change of PDV production improvement related to novel SV structures that are quantitated by HPLC. Values are averages from three experiments, and error bars denote s.d

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