Fig. 1 | Nature Communications

Fig. 1

From: Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Fig. 1The alternative text for this image may have been generated using AI.

Bidirectional flow of cerebrospinal fluid in the central canal of the spinal cord. a Single frames from time lapses of 24 h post fertilization (hpf) Tg(β-actin:Arl13B-GFP) embryos expressing GFP in cilia. Images taken from progressively dorsal focal planes. Scale: 25 µm. b Quantification of ciliary motion from time lapses; ventral planes exhibit more ciliary motion than dorsal planes (one-way ANOVA F = 12.2, p = 5.92 × 10‒7, see Methods for motion index quantification) SC spinal cord, CC central canal. c Lateral view of the central canal in transmitted light (top) and filled with fluorescent beads (bottom). Scale: 20 µm. d Schematic of horizontal slices taken for kymograph analysis for velocities of beads or endogenous particles. e Representative kymographs of beads in the central canal. Analyzed trajectories are in red (dorsal) or blue (ventral). f Imaging setup for full-field optical coherence tomography (FF-OCT). g Lateral view of the central canal showing endogenous particles obtained from FF-OCT. Scale: 20 µm. h Representative kymographs of endogenous particles in the central canal. Analyzed trajectories are in red (dorsal) or blue (ventral). i Velocities of exogenous particles (beads) and endogenous particles. Beads: 307 trajectories from n = 7 embryos; endogenous particles: 452 trajectories from n = 7 embryos. Error bars represent s.e.m. D dorsal, V ventral, R rostral, C caudal

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