Fig. 3
Improvement of the substrate specificity of CgTD toward bulky substrates by protein engineering. a The effect of TA and TD concentrations on the initial reaction rate of 1a to 4a (reactions were performed in duplicate with PaTA and CgTD enzymes, with 2.5 mM aldehydes, 25 mM glycine, and 50 µM PLP). b CgTD activity toward different substrates. c Docking of the PLP/3i complex into the active site of CgTD. d Docking of the PLP/3a complex into the active site of CgTD. e The surface of binding pocket. f Substrate access channel of CgTDWT. g Substrate access channel of CgTDF114A,R229T. All enzymatic assays were performed in triplicate, and error bars indicate ±s.d.
