Fig. 3
From: Mapping of histone-binding sites in histone replacement-completed spermatozoa
Analysis for H3-binding regions in total sperm and HRCS. a H3 ChIP-seq analysis for total sperm and HRCS in Hoxa gene cluster regions. Blue signal indicates the number of raw reads, which is scaled to mapped regions in each sample. Light blue boxes indicate the binding sites detected in both total sperm and HRCS. Light red boxes indicate the binding sites detected only in total sperm. b Distribution of H3 peaks detected in total sperm and HRCS (10,988 and 1320 peaks, respectively) among various genomic regions. c Profile plot for signal intensity of H3 ChIP-seq around binding sites detected in total sperm and HRCS. d Overlap of H3-binding sites (top) or H3 target genes (bottom) between total sperm and HRCS. e Box plot for o/e ratio in all CpG islands or the CpG islands overlapping with H3-binding sites in total sperm or HRCS. The number of CpG islands overlapping with H3-binding sites in total sperm or HRCS is shown at the bottom. The elements in box plot indicate following value; center line, median; box limits, upper and lower quartiles 1.5x interquartile range; and points, outliers. P value was calculated by Wilcoxon rank-sum test
