Fig. 3
Volume and proximity measurements across the α-globin locus in WT mice. a Gene map and locations of the BAC FISH probes to scale, with genomic distances encompassed. b Representative images for the three BAC probe pairs in MFL 30 h erythroblasts with nuclei delineated (white dotted line). Scale bar 2 μm. c Median inter-centroid distances between the three probe pairs indicated, in mES cells (grey) and erythroblasts at 0 h (purple) and 30 h (red) timepoints. MFL1, 2 and 3 represent cultures from three individual foetal livers. Light blue shading emphasises proximity of the flanking regions F1–F2 in erythroblasts. n = 87–236 — see Supplementary Fig. 3 for the complete data set. d Cumulative frequency plots of BAC signal volumes in mES cells (grey) and erythroblasts at 0 h (purple) and 30 h (red). COMP values indicating expanded volume are arrowed. All P values are derived by a Kruskal–Wallis test with Dunn’s multiple comparisons. ns not significant
