Fig. 4

Proximity measurements at the α-globin locus in mouse WT cells. a Gene map with plasmid FISH probe locations showing the pairwise combinations used to measure inter-probe distances. Using probe A as a point of reference, measurements were made to the domain side (E, Ex) of probe A compared to the control non-interacting side (C, Cx). Genomic distances between midpoints of the probe pairs are shown. b Representative images of RASER-FISH hybridisation signals for the four plasmid probe pairs in MFL 30 h erythroblasts. White dotted line delineates nuclei. Scale bar 2 μm. c Median inter-centroid distances measured between the four probe pairs in three different cell types, mES1–3 (grey), MFL4–5 0 h (purple) and MFL4-5 30 h (red). p values, derived by a Kruskal–Wallis test with Dunn’s multiple comparisons, are shown. See Supplementary Fig. 4 for full data with statistical analyses. At MFL 0 h and 30 h but not mES, the distance between A and Ex is consistently statistically shorter (p < 0.0001) than A to Cx