Fig. 5
Super-resolution imaging of the α-globin domain. a Gene map with FISH probe Ex, COMP and A locations marked against NG Capture-C tracks depicting interactions at MFL 30 h from the viewpoints CTCF BS -39.5 (black) and Hba1/2 promoters (lime green). b 2D STED maximum intensity projection images of FISH probes A and Ex (both red) which flank the α-globin domain, the COMP BAC (green) defining the extent of the domain and a merged image showing a cloud of domain signal distinct from the paired probes A and Ex. Scale bar 2 μm. c Three-colour 3D STED maximum intensity projection images of oligonucleotide probe pools (oEx, oCOMP and oA) designed to match the probe set shown in a. The oCOMP probe (blue) detects a cloud of signal that is distinct from the adjacent probes oEx (red) and oA (green). Scale bar 0.5 μm. d A drawing describes the angle measured in Euclidean 3D space between vectors joining the oCOMP signal with oEx and oA. The angles measured are charted on the right, with a median of 42°, well below an expected 60° for a random distribution. n = 28
