Fig. 8
The α-globin domain still forms in the absence of elements critical for α-globin expression. a Pairwise inter-centroid distances between three BAC probes in 30 h erythroblasts derived from littermates MFL3 (WT) and DKO1 (homozygous deletions for MCS-R1/R2). F1–F2 are significantly closer than COMP-F1 and COMP-F2 in both WT (p < 0.0001 for both) and DKO1-derived erythroblasts (p = 0.0374 and 0.0468 respectively). Total number of measurements is indicated by ‘n’. b Cumulative frequency plots of BAC signal volumes in 30 h erythroblasts from MFL3 and DKO1. The larger COMP volumes are arrowed. Total number of measurements is indicated by ‘n’. c Median inter-centroid distances between four plasmid probe pairs at MFL 0 h and 30 h from littermates WT MFL4 and two homozygous double knockout embryos DKO2 and DKO3. Light blue shading emphasises the shorter distances within the self-interacting domain in both WT and knockouts. See Supplementary Fig. 6 for the complete data set. d Median inter-centroid distances between plasmid probe pairs A-Ex (represented as A-E distance because of a 16 kb α-globin gene deletion) and A-C at MFL 30 h in two α-globin knockout lines from littermates AMKO1 and AMKO2, plotted against WT MFL4. Light blue shading is as for c. See Supplementary Fig. 6 for full data. All p values are derived by a Kruskal–Wallis test with Dunn’s multiple comparisons. ns not significant
