Fig. 1

NK cluster gene expression in each iNKT subset. a Murine NK cluster genes, including the Cd69 gene, are located on murine chromosome 6 (left). The mRNA expression of NK cluster genes in NKT1 (CD3⁺ CD1d.PBS57^low CD138^– CD44^hi), NKT2 (CD3⁺ CD1d.PBS57^hi CD138^–) and NKT17 (CD3⁺ CD1d.PBS57⁺ CD138⁺) cells sorted from BALB/c thymus and analyzed by quantitative RT-PCR. Data are shown relative to the Rpl13a expression (right). b A flow cytometry analysis of the frequencies of CD94⁺ or NKG2D⁺ cells gated on each iNKT subset (identified as in Supplementary Fig. 1c) from BALB/c thymus. c The CD69 expression on each iNKT subset (identified as in Supplementary Fig. 1c) from BALB/c thymus presented as the mean fluorescence intensity relative to that on preselection (TCRβ^lo-med CD4⁺CD8⁺) thymocytes. The mean and SEM are shown. *p < 0.05 and ***p < 0.001 (one-way ANOVA). Representative data from five experiments with five mice are shown (a). Data are pooled from two experiments with six mice (b) and three experiments with four mice (c). n.s. not significant