Fig. 5
Prevention of S1P1 surface expression rescues NKT2 cell differentiation. a Profiles of iNKT cells in the thymus from Cd69+/+, Cd69–/–, S1pr1–/–, Cd69–/–S1pr1–/– C57BL/6-background mice (left). The total frequencies of iNKT cells in the thymus are shown (right). b The frequencies of NKT1 (PLZFloT-bet+), NKT2 (PLZFhiRorγtmed), and NKT17 (PLZFmedRorγthi) cells in the thymus (upper) and among total iNKT cells (bottom) are shown. c The CD5 expression on each stage of cells presented relative to that on preselection thymocytes (DP). d Profiles of iNKT cells in the thymus from Cd69+/+ and Cd69–/– BALB/c-background mice after 5 consecutive daily injections with either saline (C) or the S1P1 agonist FTY720 (F). The total frequency of iNKT cells in the thymus is shown. e The frequencies of NKT1 (PLZFloT-bet+), NKT2 (PLZFhiRorγtmed), and NKT17 (PLZFmedRorγthi) cells in the thymus (upper) and among total iNKT cells (bottom) are shown. f The CD5 expression on each stage of NKT cells determined as in Fig. 2a and presented relative to that on preselected thymocytes. The mean and SEM are shown. *p < 0.05, **p < 0.01, and ***p < 0.001 (two-tailed unpaired t test). Representative data from 7 to 12 experiments with more than 10 mice are shown (a). Data pooled from 7 to 12 experiments with more than 10 mice (b), 2–4 experiments with more than 5 mice (c), and 3 experiments with 3–5 mice are shown (d–f). n.s. not significant
