Fig. 8
LEM4 mediated the phosphorylation of ERα-Ser167 by Aurora-A. a Immunoblot analysis of the phosphorylation of Aurora-A, Aurora-A, AKT, and S6K1 in MCF7-LEM4 and MCF7-TAMR cells. b LEM4 siRNA-treated and control siRNA-treated MCF7-LEM4 and MCF7-TAMR cells were treated for 48 h. Western blot was performed with indicated antibodies. c BT474 cells were transfected with LEM4 siRNA or control siRNA for 48 h. Western blot was performed with indicated antibodies. d MCF7-LEM4 cells were transfected with LEM4 siRNA or AKT siRNA or Aurora-A siRNA for 48 h. Western blot was performed with indicated antibodies. e HEK293T cells were transfected with FLAG-LEM4 and GFP-Aurora-A. After incubating for 48 h, cell lysates were precipitated with anti-FLAG affinity gel and immunoblotted with anti-GFP antibody. f HEK293T-shLEM4 cells were transfected with FLAG-ERα and GFP-Aurora-A. After incubating for 48 h, cell lysates were precipitated with anti-FLAG affinity gel and immunoblotted with anti-GFP antibody. g MCF7-shControl and MCF7-shLEM4 cells, or BT474-shControl and BT474-shLEM4 cells were treated with 50 μg mL−1 CHX for 0, 1, 2, and 4 h and analyzed for Aurora-A by immunoblot. h Model of LEM4 regulation of the ERα signaling leading to tamoxifen resistance in ER+ breast cancer
