Fig. 4
From: High-resolution NMR studies of antibiotics in cellular membranes
The nisin:lipid II pore topology. a Membrane arrangement of the nisin:lipid II topology as seen by ssNMR. Plastic residues that are required to adapt to the bacterial target membrane are highlighted with red circles. Residues that showed 1H/2H exchange are colored in blue and align the pore lumen. The C terminus is dynamically disordered and resides at the water–membrane interface. The A–B rings (in magenta) interact with the lipid II PPi group. b Residues I432, K1238, N20–K2215, 16, 20, 21, 30, and S2954 are pharmaceutical hotspots that enable to improve nisin’s activity upon mutation. These residues were all identified as important for nisin’s cellular adaptability in this study
