Fig. 1
From: Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
Manual vs. single-step deposition by microfluidic spray device. AFM sample preparation by (a) conventional manual deposition. This method may cause the (b) self-organization of monomeric α-synuclein along the crystallographic lattice of the mica substrate (scale bar 100 nm). c Furthermore, manual deposition typically enables only a partial depiction of a heterogeneous Aβ42 aggregated solution on a mica surface because of differential adsorption (scale bar 100 nm). While, (d) single-step microfluidic spray deposition (e) conserves the molecular architecture and assembly state of proteins in solution, and (f) enables to unravel the full content of the multiple protein species in solution (scale bars 200 nm)
