Fig. 4
From: Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
Microfluidic spray deposition avoids artificial self-assembly and enables accurate tuning of molecules concentration on the surface. AFM maps of the microfluidic spray deposition of a monomeric solution of α-synuclein at (a) 0.2 μM, (b) 2 μM, and (c) 3 μM for 5 s at 100 μl h−1 (scale bars 200 nm). d Box chart distributions of the cross-sectional height of the deposited biomolecules is independent from the concentration of deposition (n = 1003 0.2 μM, n = 2441 for 0.5 μM, n = 3685 for 3 μM; the horizontal line represents the median, the box the first to third interquartile, and the error is the s.d.). e Average molecular distance on the surface (average nearest neighbor) decreases as a function of the concentration of the deposited solution, in excellent agreement with theoretical calculations (values are mean ± s.d.)
