Fig. 5

Spray deposition avoids selective sample adsorption. a Kinetic profiles of the aggregation reaction of 10 μM Aβ40 under standard conditions (blue curve) and in the presence of protoporphyrin (Proto), which inhibits its aggregation (brown curve). Deposition of the final products of the aggregation in the absence and presence of the inhibitor by (b) manual (scale bar: top image 1000 nm, bottom image 200 nm) and (c) automated spray deposition (scale bar: top image 1000 nm, bottom image 200 nm). d Illustration of the differences between the two preparative methods and the effects leading to selective adsorption. Quantification of the absolute mass (values are mean ± s.d.) of coexisting amyloid species at the end of the aggregation reaction by measuring the volume of each species in (e) standard and (f) inhibited conditions of aggregation